Hordhaca midabka DPD

DPD spectrophotometry waa habka caadiga ah ee lagu ogaanayo koloriinta hadhaaga ah ee bilaashka ah iyo wadarta koloriin haraaga ah ee halbeegga qaranka Shiinaha "Erayada Tayada Biyaha iyo Hababka Falanqaynta" GB11898-89, oo ay si wadajir ah u soo saareen Ururka Caafimaadka Dadweynaha Mareykanka, Ururka Shaqada Biyaha Mareykanka iyo Xakamaynta Wasakhowga Biyaha Federaalka. Tafatirka "Habka Tijaabada caadiga ah ee Biyaha iyo Biyaha", habka DPD ayaa la sameeyay tan iyo daabacaaddii 15aad waxaana lagula talinayaa habka caadiga ah ee lagu baadho koloriin oksijiinta.
Faa'iidooyinka habka DPD
Waxay kala sooci kartaa chlorine dioxide iyo noocyo kale duwan oo koloriin ah (oo ay ku jiraan koloriin haraaga bilaashka ah, wadarta koloriin haraaga ah iyo chlorite, iwm.), taasoo sahlaysa samaynta tijaabooyinka midabaynta. Habkani maaha mid sax ah sida titration amperometric, laakiin natiijadu waa ku filan inta badan ujeedooyinka guud.
mabda'a
Marka la eego shuruudaha pH 6.2 ~ 6.5, ClO2 waxay marka hore la falgashaa DPD si ay u sameyso xarun cas, laakiin qadarku wuxuu u muuqdaa kaliya inuu gaaro shan meelood meel ka mid ah wadarta guud ee koloriinta la heli karo (oo u dhiganta dhimista ClO2 ilaa ions chlorite). Haddii muunadda biyuhu ay aashitada ku jirto iyadoo ay joogaan iodide, chlorite iyo chlorate ayaa sidoo kale falceliya, iyo marka la dhexdhexaadiyo marka lagu daro bicarbonate, midabka ka soo baxa wuxuu u dhigmaa wadarta guud ee koloriinta la heli karo ee ClO2. Faragelinta koloriinta bilaashka ah waxaa lagu joojin karaa iyadoo lagu darayo glycine. Saldhigga ayaa ah in glycine ay isla markiiba u rogi karto koloriinta bilaashka ah ee koloriinta aminoacetic acid, laakiin wax saameyn ah kuma laha ClO2.
Potassium iodate xal saamiga caadiga ah, 1.006g/L: Miisaanka 1.003g potassium iodate (KIO3, la qalajiyey at 120 ~ 140 ° C 2 saacadood), milaan biyo-nadiifinta sare, iyo wareejin ilaa 1000ml mugga.
Ku walaaq weelka cabbiraadda calaamadda oo qas.
Xalka caadiga ah ee potassium iodate, 10.06mg/L: Qaado 10.0ml ee xalalka kaydka ah (4.1) ee galaaska mugga 1000ml, ku dar qiyaastii 1g oo ah potassium iodide (4.5), ku dar biyo si aad u milmaan calaamadda, oo isku qas. U diyaari maalinta isticmaalka dhalada brown 1.00ml ee xalkan caadiga ah waxa uu ka kooban yahay 10.06μg KIO3, taas oo u dhiganta 1.00mg/L koloriin la heli karo.
Fosfate kaydka: milmi 24g anhydrous disodium hydrogen phosphate iyo 46g anhydrous potassium dihydrogen fosfate biyo diiray, ka dibna ku qas 100ml oo biyo nadiif ah 800mg EDTA cusbada kala diri. Ku qas biyo la miiray ilaa 1L, ikhtiyaar ahaan ku dar 20mg chloride mercuric ama 2 dhibcood oo toluene ah si looga hortago korriinka caaryada. Ku darista 20 mg oo koloriin meerkurid ah waxay meesha ka saari kartaa faragelinta qaddarka iodide-ka ee ku hadhi kara marka la cabbirayo koloriin bilaash ah. (Fiiro gaar ah: Mercury chloride waa sun, si taxadir leh u ilaali kana fogow in la nuugo)
N,N-diethyl-p-phenylenediamine (DPD) Tusiyaha: Milki 1.5g DPD sulfate pentahydrate ama 1.1g anhydrous DPD sulfate oo ku jira biyo saafi ah oo bilaasha ah oo ka kooban 8ml1+3 sulfuric acid iyo 200mg EDTA cusbo disodium, bakhaar 1 litir ku rid dhalo dhalada dhulka brown ah, oo ku kaydi meel mugdi ah. Marka tilmaamuhu dhumo, waxay u baahan tahay in dib loo dhiso. Si joogto ah u hubi qiimaha nuugista ee shaybaarrada bannaan,
Haddii qiimaha nuugista ee bannaan ee 515nm uu dhaafo 0.002/cm, dib u habeyntu waxay u baahan tahay in laga tago.
Potassium iodide (KI crystal)
Sodium arsenite Xalka: Ku mil 5.0g NaAsO2 biyo saafi ah kuna qas 1 litir. Fiiro gaar ah: NaAsO2 waa sun, iska ilaali in la nuugo!
Xalka Thioacetamide: Ku milmi 125 mg of thioacetamide 100 ml oo biyo nadiif ah.
Xalka Glycine: Ku mil 20g glycine oo biyo ah oo aan koloriin ku jirin kuna qas 100ml. Bakhaarka waa la qaboojiyey Waxa loo baahan yahay in dib loo dhiso marka turbidity dhaco.
Xalka Sulfuric acid (qiyaastii 1mol/L): Ku mil 5.4ml H2SO4 oo la ururiyey 100ml biyo saafi ah.
Xalka Sodium hydroxide (qiyaastii 2mol/L): Miisaam 8g NaOH oo ku mil 100ml biyo saafi ah.
Qallooca (shaqaynta) qalooca
Si taxane ah oo 50 tuubbo midab leh, ku dar 0.0, 0.25, 0.50, 1.50, 2.50, 3.75, 5.00, 10.00ml of potassium iodate solution standard, siday u kala horreeyaan, ku dar 1g oo ah potassium iodide iyo 0.5ml oo ah xal sulfuric ah, istaag 2 daqiiqo, ka dibna ku dar 0.5ml sodium hydroxide xal oo ku milo calaamadda. Isku-duubnaanta dhalada kasta waxay u dhigantaa 0.00, 0.05, 0.10, 0.30, 0.50, 0.75, 1.00, iyo 2.00 mg/L ee koloriin la heli karo. Ku dar 2.5ml oo ah bakhaarka phosphate iyo 2.5ml ee xalka tusaha DPD, si fiican isku qas, isla markiiba (2 daqiiqo gudahood) ku cabbir nuugista 515nm adigoo isticmaalaya cuvette 1-inch ah. Sawir qalooca caadiga ah oo hel isla'egta regression.
Tallaabooyinka go'aaminta
Chlorine dioxide: Ku dar 1ml oo glycine ah 50ml oo muunad biyo ah oo walaaq,kadib ku dar 2.5ml ee fosfat buffer iyo 2.5ml ee tusaha DPD, si fiican isku qas, oo isla markiiba cabbir nuugista (2 daqiiqo gudahood) (akhrisku waa G).
Chlorine dioxide iyo koloriin bilaash ah oo la heli karo: Qaado muunad biyo ah oo 50ml oo kale ah, ku dar 2.5ml phosphate buffer iyo 2.5ml tusaha DPD, si fiican isku qas, oo isla markiiba cabbir nuugista (2 daqiiqo gudahood) (akhrisku waa A).
7.3 Chlorine dioxide, koloriin bilaash ah oo la heli karo iyo koloriin la isku daray oo la heli karo: Qaado 50ml kale oo muunad biyo ah, ku dar ilaa 1g oo potassium iodide ah, ku dar 2.5ml ee phosphate buffer iyo 2.5ml ee tusaha DPD, si fiican isku qas, oo isla markiiba cabbir nuugista 2 daqiiqo) (Akhrisku waa C).
Wadarta koloriin la heli karo oo ay ku jiraan koloriin dioxide bilaash ah, koloriin, koloriin hadhaa ah oo bilaash ah iyo koloriin hadha oo la isku daray: Ka dib markaad hesho akhrinta C, ku dar 0.5ml xal sulfuric acid muunadda biyaha ee isla dhalada midabaysan, oo isku qas Ka dib markaad istaagto 2 daqiiqo, ku dar 0.5 ml sodium hydroxide xal, qas oo qiyaas nuugista isla markiiba (akhrinta waa D).
ClO2=1.9G (loo xisaabiyay sida ClO2)
Koloriin bilaash ah oo la heli karo=AG
Koloriin la heli karo oo la isku daray = CA
Wadarta koloriinta la heli karo=D
Chlorite=D-(C+4G)
Saamaynta Manganese: Walaxda faragelinta ugu muhiimsan ee la kulma biyaha la cabbo waa manganese oxide. Ka dib marka lagu daro fosfooraska fosfooraska (4.3), ku dar 0.5 ~ 1.0ml sodium arsenite solution (4.6), ka dibna ku dar tilmaame DPD si aad u cabbirto nuugista. Ka jar akhrintan akhrinta A si aad u tirtirto
Ka saar faragelinta manganese oxide.
Saamaynta heerkulka: Dhammaan hababka falanqaynta ee hadda jira ee kala saari kara ClO2, koloriin bilaash ah iyo koloriin isku dhafan, oo ay ku jiraan titration amperometric, habka iodometric joogtada ah, iwm., heerkulku wuxuu saameyn doonaa saxnaanta farqiga. Marka heerkulku sarreeyo, koloriin la isku daray (chloramine) ayaa lagu soo dayici doonaa inay ka qaybqaataan falcelinta hore, taas oo keenta natiijooyin sare oo ClO2 ah, gaar ahaan koloriin bilaash ah. Habka ugu horreeya ee xakamaynta waa in la xakameeyo heerkulka. Qiyaastii 20°C, waxa kale oo aad ku dari kartaa DPD muunada biyaha oo isku qas, ka dibna isla markiiba ku dar 0.5ml thioacetamide (4.7) si aad u joojiso isku darka chlorine (chloramine) ee DPD. Falcelinta
Saamaynta wakhtiga midabaynta: Dhinac, midabka cas ee ay soo saartay ClO2 iyo tilmaame DPD waa mid aan degganayn. Midabka mugdiga ah, si dhakhso ah ayuu u libdhiyaa. Dhanka kale, sida xalka fosfatetada iyo tusaha DPD ay isku qasan yihiin waqti ka dib, iyaga laftoodu sidoo kale way libdhi doonaan. Waxay soo saartaa midab casaan ah oo been ah, waayo-aragnimaduna waxay muujisay in wakhtigan ku-tiirsanaanta midab-darrada ah ay tahay sababta ugu weyn ee hoos u dhaca saxnaanta xogta. Sidaa darteed, dedejinta tallaabo kasta oo hawlgal ah iyada oo la xakameynayo jaangooyooyinka wakhtiga loo isticmaalo tallaabo kasta ayaa muhiim u ah hagaajinta saxnaanta. Sida laga soo xigtay waayo-aragnimada: horumarinta midabka ee ka hooseeya 0.5 mg / L waxay noqon kartaa mid deggan ilaa 10 ilaa 20 daqiiqo, korriinka midabka ee qiyaasta 2.0 mg / L wuxuu noqon karaa oo kaliya ilaa 3 ilaa 5 daqiiqo, iyo korriinka midabka ee ka sarreeya 5.0 mg/L wuxuu ahaan doonaa mid deggan wax ka yar 1 daqiiqo.
TheLH-P3CLOHadda ay bixiso Lianhua waa la qaadi karomitirka koloriin ee haraaga ahkaas oo u hogaansama habka sawir-qaadista ee DPD.
Falanqeeyaha ayaa horey u dejiyay hirarka dhererka iyo qalooca. Kaliya waxaad u baahan tahay inaad ku darto reagen oo aad sameyso midabaynta si aad dhakhso ugu hesho natiijooyinka koloriin haraaga ah, wadarta koloriin haraaga ah iyo koloriin dioxide ee biyaha ku jira. Waxa kale oo ay taageertaa sahayda batteriga iyo korontada gudaha, taas oo sahlaysa in la isticmaalo dibadda ama shaybaadhka.